【文獻(xiàn)標(biāo)題】Isolation and identification of senescent renal tubular epithelial cells using immunomagnetic beads based on DcR2
【作者】Jia Chen,Ke-Hong Chen,Bi-Qiong Fu,et.al
【作者單位】第三軍醫(yī)大學(xué)(Third Military Medical University)
【文獻(xiàn)中引用產(chǎn)品】
人白細(xì)胞介素6(IL-6)ELISA試劑盒
人轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)ELISA試劑盒
【關(guān)鍵詞】Cell senescence,Renal tubular epithelial cell,Cell sorting,Decoy receptor 2
【DOI】dx.doi.org/10.1016/j.exger.2017.04.008
【影響因子(IF)】5.10
【出版期刊】《Experimental Gerontology》
【產(chǎn)品原文引用】
Cell supernatants were collected from the control group, passage 5,AGEs and H2O2 groups. After the adherence of MACS-sorted DcR2-positive and negative cells, normal medium was added. The cell supernatant of the two groups was collected after 48 h. The levels of IL-6 and TGF-β1 in the supernatant were measured by ELISA kit (Hengyuan,Shanghai, China), according to the manufacturer's instructions.
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